Whether or not the great Italian violin-makers used wood that had been chemically processed in order to preserve it and enhance the instrument's sound quality has long been a contentious issue1, 2. Here we use nuclear magnetic resonance and infrared spectroscopy to analyse organic matter in wood taken from antique instruments made by Stradivari and Guarneri del Gesu. Our results indicate that the wood used by the masters could indeed have been chemically treated, a technique that may inspire an approach to violin making that is more chemistry-based.